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| Registros recuperados: 49 | |
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| Souza Júnior,Manoel Teixeira; Gonsalves,Dennis. |
| The Papaya ringspot virus (PRSV) coat protein transgene present in 'Rainbow' and 'SunUp' papayas disclose high sequence similarity (>89%) to the cp gene from PRSV BR and TH. Despite this, both isolates are able to break down the resistance in 'Rainbow', while only the latter is able to do so in 'SunUp'. The objective of this work was to evaluate the degree of sequence similarity between the cp gene in the challenge isolate and the cp transgene in transgenic papayas resistant to PRSV. The production of a hybrid virus containing the genome backbone of PRSV HA up to the Apa I site in the NIb gene, and downstream from there, the sequence of PRSV TH was undertaken. This hybrid virus, PRSV HA/TH, was obtained and used to challenge 'Rainbow', 'SunUp', and an... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carica papaya; Potyvirus; Resistance; Breeding; Virus. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000500009 |
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| Aguiar,Raimundo Wagner de Souza; Rodrigues,Adelmo; Portella,Augustus Caeser Franke; Lopes,Magnolia de Mendonça; Lima,Mirtes Freitas; Resende,Renato de Oliveira; Nagata,Tatsuya. |
| Watermelon (Citrullus lanatus) cultivated in almost all tropical and subtropical regions of the world, has its largest output in China, and then, according to FAO data, Turkey, Iran and Brazil, being one of the main crops cultivated in State of Tocantins, Brazil. In this work was investigated the occurrence and distribution of the watermelon viruses, totaling 752 samples taken in a stratified experimental design in four representative regions of production: Gurupi (150), Lagoa da Confusao (232), Formoso do Araguaia (265) and Porto Nacional (105). The sampling and collecting the leaves of plants with the presence of symptoms were performed once a week during the entire cultivation cycle. As a result, were observed by Dot-ELISA method, different types of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Watermelon; Citrullus lanatus; Potyvirus; Cucumovirus; Tospovirus; Serological assay. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200192 |
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| Maciel-Zambolim,Eunize; Costa,Helcio; S. Capucho,Alexandre; Ávila,Antônio C. de; Inoue-Nagata,Alice K.; Kitajima,Elliot W.. |
| Este trabalho relata a ocorrência de um surto epidemiológico causado pelo vírus do mosaico amarelo do pimentão (Pepper yellow mosaic virus - PepYMV) em tomateiro (Lycopersicon esculentum) 'Alambra' na região serrana do Estado do Espírito Santo. Os sintomas consistiam de mosaico, definhamento e redução de produção. Visando a caracterização do agente causal foram realizados estudos sorológicos por ELISA, observações ao microscópio eletrônico e determinação da gama parcial de hospedeiros. Ao microscópio eletrônico de transmissão foram observadas, em amostras de tomateiro, partículas alongadas e flexuosas e inclusões cilíndricas típicas de vírus do gênero Potivirus. O PepYMV foi confirmado como agente causal por ELISA indireto. Levantamentos realizados em... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lycopersicon esculentum; Potyvirus; Pepper yellow mosaic virus. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000300017 |
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| Mituti,Tatiana; Moura,Mônika Fecury; Marubayashi,Julio Massaharu; Oliveira,Milena Leite; Imaizumi,Vitor Massami; Sakate,Renate Krause; Pavan,Marcelo Agenor. |
| Garlic (Allium sativumL.) is a host to several viruses, most commonly those belonging to theAllexivirus,Carlavirus, orPotyvirusgenera. Nine species distributed among these three genera have been reported in Brazil: two species within carlaviruses, two within potyviruses, and five within allexiviruses. To quantify the prevalence of these viruses, young leaves from 520 plants (plants either symptomatic or asymptomatic) were collected from commercial fields grown in four Brazilian states and analyzed using universal and species-specific primers via the reverse transcription polymerase chain reaction (RT-PCR). Potyvirus presence was positive in 306 samples (81 %), 151 of them (38 %) in mixed infections with other viruses. The most frequent potyviruses... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allexivirus; Allium sativum; Carlavirus; Potyvirus; Occurrence. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000300278 |
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| Alfenas,Poliane F.; Braz,Antonio Sérgio K.; Torres,Leonardo B.; Santana,Enilton N.; Nascimento,Ana Verônica S. do; Carvalho,Murilo G. de; Otoni,Wagner C.; Zerbini,F. Murilo. |
| Sixteen transgenic yellow passionfruit (Passiflora spp.) plants (R0) were obtained which express a non-translatable transgenic RNA corresponding to the 3' region of the NIb gene and the 5' region of the CP gene, derived from the genome of a Brazilian isolate of Cowpea aphid-borne mosaic virus (CABMV). The transgenic plants were propagated by stem cuttings and challenged by sap inoculation with isolates CABMV-MG1 and CABMV-PE1. One transgenic plant (TE5-10) was resistant to the isolate CABMV-MG1, but susceptible to CABMV-PE1. The remaining transgenic plants developed systemic symptoms, equal to non-transformed plants, when inoculated with either isolate. The absence of virus in TE5-10 plants was confirmed by indirect ELISA. Transcription analysis of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Passionfruit woodiness virus; PWV; Potyvirus; Resistance. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000100006 |
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| Bonilha,Estela; Gioria,Ricardo; Kobori,Rômulo Fujito; Vecchia,Paulo Tarcísio Della; Piedade,Sônia Maria de Stefano; Rezende,Jorge Alberto Marques. |
| Papaya ringspot virus - type W (PRSV-W) and Zucchini yellow mosaic virus (ZYMV) are the most prevalent viruses in cucurbit crops in Brazil and responsible for frequent yield losses. Diseases caused by these viruses are difficult to control. The objective of this work was to evaluate the effects of mild strains PRSV-W-1 and ZYMV-M on the yield of Cucurbita pepo L. cvs. Samira, Novita Plus, AF 2847, and Yasmin, under plastic greenhouse and field conditions. Plants infected with ZYMV-M and grown in a plastic greenhouse did not exhibit typical leaf symptoms or significant alterations in quantitative and qualitative fruit yield. However, when infected with PRSV-W-1, or PRSV-W-1 + ZYMV-M, the plants exhibited severe leaf mosaic symptoms and reduced fruit... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Control; Potyvirus; Cross protection; Cucurbitaceae. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000300019 |
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| Registros recuperados: 49 | |
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